Coding

Part:BBa_K5131000:Design

Designed by: Yu Han   Group: iGEM24_Squirrel-Shanghai   (2024-09-30)


SARA-Cov-2 nsp5


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 390


Design Notes

We used JCat for codon optimization of the sequence to ensure efficient expression in E. coli, and added a 6xHis tag at the C-terminus to facilitate protein purification.


Source

SARS-CoV-2 Wuhan-hu-1(NC_045512)

References